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. 2023 Feb 15;15(1):71–81. doi: 10.1007/s12560-022-09542-z

Table 4.

Quality parameters for the RT-qPCR assays based on the Fast Virus (FV) and qScript (qS) chemistries used in this study showing±1 standard error of the mean

Quality parameter FV qS
R2 0.998 ± 0.000 0.999 ± 0.000
Slope (Cq) −3.304 ± 0.020 −3.407 ± 0.019
Intercept (Cq) 37.578 ± 0.119 37.855 ± 0.146
Amplification efficiency (%) 100.858 ± 0.903 96.66 ± 0.927