Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 25;222(3):e202204102. doi: 10.1083/jcb.202204102

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Berman et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 5. — Microtubule foci formation and coalescence in live γ-tubulin^ΔGTP+KD cells. (A–E) Live imaging of microtubule regrowth assay by spinning disc confocal microscopy in γ-tubulin^KD (A; no GFP signal), γ-tubulin^WT+KD (B and C), and γ-tubulin^ΔGTP+KD (D and E) cells. Maximum-intensity projections at individual time points are shown. Timestamps = hh:mm. Single-channel images (A, B, D) show SiR–tubulin-labeled microtubules. Overlays (C and E) show GFP-tagged γ-tubulin (green) and SiR–Tubulin-labeled microtubules (magenta). Scale bar = 2.5 µm. (F) Quantification of the number of microtubule foci in γ-tubulin^KD (dark blue bars, n = 18 total cells from N = 2 independent experiments), γ-tubulin^WT+KD (gray bars, n = 13 total cells from N = 3 independent experiments), and γ-tubulin^ΔGTP+KD (light blue bars, n = 23 total cells from N = 3 independent experiments) cells.