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. Author manuscript; available in PMC: 2023 Nov 16.
Published in final edited form as: Nature. 2022 Nov 16;611(7937):818–826. doi: 10.1038/s41586-022-05432-3

Extended Data Figure 9. TCR from Pt 1 exPBMC recognizes α-myosin epitope.

Extended Data Figure 9.

a) TCR-Pt1, which was cloned and transduced into Jurkat NFAT-GFP reporter cells, is shown in red on the same plot show in Fig 4c. This shows the expansion of this TCR in the exPBMC and abundance in the heart. b) Representative flow cytometry scatter plots are shown for the TCR-pt1 Jurkat cell line is stained with A*24:02 tetramer with RINATLETK or A*03:01 tetramer with RINATLETK. c) Full flow cytometry gating strategy for human PBMC and exPBMC tetramer staining. Debris, doublets and dead cells (Zombie Violet positive) are excluded. CD3+CD8+ cells are used for tetramer analysis. Tetramer staining for all samples is shown.