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. Author manuscript; available in PMC: 2023 Nov 16.
Published in final edited form as: Nature. 2022 Nov 16;611(7937):818–826. doi: 10.1038/s41586-022-05432-3

Extended Data Figure 5. Thymic expression of Myh6 and flow cytometry gating for murine α-myosin tetramers.

Extended Data Figure 5.

a) Gene expression for Myh6, Nppa, Nppb, and Sbk2 in the heart and thymus of n=3 each male and female Pdcd1−/−Ctla4+/− mice. Gene expression is normalized to beta-actin. Gene expression is plotted as 2^-(Ct gene of interest minus Ct of beta-actin). Box plots show the median, first and third quartiles. The whiskers extend to the maxima and minima but no further than 1.5 times the inter-quartile range. b) Gating strategy for H2-Kb tetramers on murine heart samples. Debris, doublets and dead cells (Zombie Violet positive) are excluded. CD3+CD8+ cells are used for tetramer analysis. Staining for Control (SIINFEKL) H2-Kb, and VQQVYYSI H2-Kb tetramers are shown. c) Quantification of spleen tetramer positive CD3+CD8+ cells, by sex of the mouse. The spleens used in this experiment correspond to the mice show in Fig. 3f, which all have α-myosin tetramer positive MC.