Table 1.
Impact of G29179T mutation on ΔCt in two PCR assays.
| Mutation | Sample No. | Xpert Xpress SARS-CoV-2 |
Allplex™ SARS-CoV-2 Assaya |
Novaplex™ SARS-CoV-2 Variant Ⅰ Assay | ||||
|---|---|---|---|---|---|---|---|---|
| E (Ct) | N2 (Ct) | ΔCt | E (Ct) | N (Ct) | ΔCt | |||
| G29179T (−) | 20 | 28.2 | 31.3 | 3.1 | 29.6 | 28.0 | −1.7 | (R.1) |
| 23 | 12.8 | 14.9 | 2.1 | 9.7 | 9.6 | −0.1 | (R.1) | |
| 24 | 30.3 | 32.6 | 2.3 | 31.3 | 28.9 | −2.3 | (R.1) | |
| 25 | 25.3 | 27.3 | 2.0 | 26.8 | 23.9 | −2.9 | alpha variant (B.1.1.7) | |
| 27 | 23.9 | 25.3 | 1.4 | 25.0 | 23.7 | 1.2 | alpha variant (B.1.1.7) | |
| 29 | 17.1 | 18.2 | 1.1 | 18.1 | 16.3 | 1.8 | alpha variant (B.1.1.7) | |
| 31 | 31.9 | 33.0 | 1.1 | 30.7 | 28.3 | 2.4 | alpha variant (B.1.1.7) | |
| G29179T (+) | 30 | 24.6 | 35.0 | 10.4 | 25.9 | 23.8 | 2.1 | alpha variant (B.1.1.7) |
| 32 | 31.9 | 44.2 | 12.3 | 33.0 | 30.9 | 2.1 | alpha variant (B.1.1.7) | |
| 33 | 22.4 | 34.1 | 11.7 | 22.9 | 21.1 | 1.8 | alpha variant (B.1.1.7) | |
| 34 | 15.1 | 25.5 | 10.4 | 15.2 | 12.8 | 2.4 | alpha variant (B.1.1.7) | |
a
Allplex™ SARS-CoV-2 Assay detects the E, N, and RdRp/S genes, but the results for the RdRp/S gene were omitted (the Ct values were similar to those for the E and N genes).