TABLE 1:
Frequency of cargo cotransport.
| Strain | DRC2-GFP (N) (ante/retro) | DRC4-mC (N) (ante/retro) | Cotransports (N) (ante/retro) | Cilia (N) | Time (s) |
|---|---|---|---|---|---|
| ida6 pf2 DRC2-GFP DRC4-mC (regenerating flagella) | 225(212/13) | 152(142/10) | 47(44/3) | 52 | 3158 |
| CC-5325 × ida6 pf2 DRC2-GFP DRC4-mC (mutant-derived flagella) | 137(130/7) | 117(107/10) | 11(9/2) | 73 | 5165 |
The table lists the observed IFT transports for DRC2-GFP and DRC4-mC in flagella of the double mutant–double rescue strain and in wild-type–derived zygotic cilia obtained by mating CC-5325 and ida6 pf2 DRC2-GFP DRC4-mC.