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. 2023 Jan 13;34(2):ar11. doi: 10.1091/mbc.E20-03-0179

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© 2023 Caydasi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — SWR1-C is not essential for SAC integrity. (A, B) SAC integrity of indicated cell types. Cells were released from a G1 block (t = 0) into nocodazole-containing medium. Samples were collected at indicated time points. (A) Percentages of large- and multibudded cells were scored and (B) Pds1-3HA, Clb2, Sic1 and Tubulin levels were analyzed by immunoblotting at the indicated time points. (C) Deletion of SWR1 does not rescue temperature-sensitive men-ts. Serial dilutions of SWR1 or swr1∆ cells carrying the indicated temperature-sensitive alleles of the genes coding for MEN proteins were grown on YPAD plates at indicated temperatures.