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. 2023 Jan 13;34(2):ar11. doi: 10.1091/mbc.E20-03-0179

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© 2023 Caydasi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — Swr1 does not affect FEAR. (A) The percentage of cells with partially released Cdc14. Cultures of indicated cell types bearing mCherry-Tubulin and Cdc14-GFP were synchronized in G1 by alpha factor treatment (t = 0) at the permissive temperature of cdc15-1 (23°C) and released in alpha factor free medium at the restrictive temperature (37°C). Samples were collected every 10 min and Cdc14 release was scored as a function of spindle length. Data are an average of three independent experiments. Error bars are SD. (B) The percentage of cells with spindle-enriched Sli15-GFP. Cultures of indicated cell types bearing mCherry-Tubulin and Sli15-GFP were synchronized in G1 by alpha factor treatment (t = 0) at the permissive temperature of cdc15-1 (23°C) and released in alpha factor free medium at the restrictive temperature (37°C). Samples were collected every 10 min, and spindle-enriched Sli15 was scored as a function of spindle length. Data are an average of three independent experiments. Error bars are SD. Scale bars: 3 µm. Two-way ANOVA test was performed in A and B. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.