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. 2023 Feb 14;11(2):e005126. doi: 10.1136/jitc-2022-005126

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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The expression of GLIS1 in PBMC CD8⁺ T cells of HCC patients predicted the immune status in tissues. (A) GLIS1 expression in CD8⁺ TILs from 10 HCC patients tissues was analyzed by flow cytometry, and were divided into high GLIS1 expression and low GLIS1 expression groups. The qRT-PCR results of the common markers of CD8⁺ T cell exhaustion (PD1, LAG3, TIM3 and CTLA4) and cytokines (IFN-γ, TNF-α) expression in each group. (B) Samples from HCC patients CD8⁺ TILs were used for immunofluorescence detection of CD8 and GLIS1 expression in each group. (C, D) The expression of GLIS1 in CD8⁺ TILs and corresponding peripheral blood CD8⁺ T cells of 40 HCC patients were analyzed by flow cytometry, respectively, and ranked them from low to high. (E, F) The correlation analysis between the GLIS1 expression in CD8⁺ TILs and PBMC CD8⁺ T cells. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. HCC, hepatocellular carcinoma; PBMC, peripheral blood mononuclear cell; qRT-PCR, quantificational real-time PCR; TILs, tumor infiltrating lymphocytes.