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. 2023 Feb 14;11(2):e005126. doi: 10.1136/jitc-2022-005126

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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GLIS1 knockdown alleviated CD8⁺ T cell exhaustion in vitro. (A, B) shRNA against GLIS1 was designed to silence GLIS1 level in CD8⁺ T cells, which was validated by qRT-PCR (A) and western blotting(B). (C) CFSE experiment showing CD8⁺ T cell proliferation ability in sh-NC and sh-GLIS1 group, respectively. (D) Flow cytometry was used to assess CD8⁺ T cell apoptosis in sh-NC and sh-GLIS1 group. (E) We measured the expressions of common exhausted markers of CD8⁺ T cells (PD1, TIM3, LAG3, CTLA4) by flow cytometry. (F) We measured the expressions of cytokines of CD8⁺ T cells (IL-2, IFN-γ, TNF-α) by flow cytometry. *p<0.05, **p<0.01, ***p<0.001. NS indicates no significant difference. CFSE, carboxyfluorescein diacetate; qRT-PCR, quantificational real-time PCR.