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. 2023 Feb 14;11(2):e005126. doi: 10.1136/jitc-2022-005126

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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CHIP and RNA sequence analysis when GLIS1 was knockdown in CD8⁺ TIL from HCC patients. (A) RNA transcriptome sequencing was used to assess the different mRNAs of sh-NC and sh-GLIS1 group. 22 and 32 represented two groups.Heatmap was presented. (B) Schematic diagram of the experimental steps of CHIP-seq. (C) CHIP sequencing was used to assess genes regulated by GLIS1 transcription. 1 and 2 represented two groups.Volcano map was presented. (D) We cross-analyzed the results of RNA sequencing and CHIP to search for common genes shared by the two sequencing. (E) Volcano plot showing cross-analyzed genes including SGK1. (F) GSEA analysis of total RNA-seq, indicating the different genes were enriched in CD8⁺ T exhaustion. (G) Pathway analysis of mRNAs in sh-NC and sh-GLIS1 group. (H) GO analysis of RNA-seq22. (I) KEGG analysis of RNA-seq22. (J) KEGG analysis of CHIP-seq. CHIP, chromatin immunoprecipitation; HCC, hepatocellular carcinoma.