Malvasi 2014.

Study characteristics
Methods	Study type: parallel RCT
Participants	65 pregnant women from Italy Inclusion criteria: healthy pregnant women, aged between 30 to 40, between 13 and 24 weeks' gestation, BMI between 25 to 30 kg/m2. Exclusion criteria: diabetes mellitus, cardiovascular disease, chronic hypertension, autoimmune disease, dysthyroidism. Location: Bari, Italy Timeframe: January to December 2012
Interventions	Intervention: a combination of 2000 mg myo‐inositol, 400 mg d‐chiro‐inositol, 400 mcg folic acid, 10 mg manganese Duration of myo‐inositol supplementation: 60 days Comparison: placebo (but not stated what placebo was)
Outcomes	Maternal: total cholesterol, LDL, HDL, blood glucose Criteria used to diagnose gestational diabetes: not stated Infant: not stated
Notes	Sample size calculation: not stated Funding source: not stated Conflicts of interest: none reported Further information was received following email contact with the authors.
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	Low risk	Randomisation sequence was generated by a random number table.
Allocation concealment (selection bias)	Low risk	Allocation was controlled by an independent statistician who assigned numbered patients to groups using sealed numbered containers.
Blinding of participants and personnel (performance bias) All outcomes	Unclear risk	Participants were blinded. Clinicians were aware of treatment allocation.
Blinding of outcome assessment (detection bias) All outcomes	Low risk	Outcome assessors were blinded.
Incomplete outcome data (attrition bias) All outcomes	High risk	65 women were initially enrolled, 17 of which were excluded; 6 did not meet inclusion criteria, 4 refused to participate, 7 left the study spontaneously. Analysis was conducted on the remaining 48 women.
Selective reporting (reporting bias)	Low risk	All pre‐specified outcomes are reported on (total cholesterol, LDL, HDL, blood glucose). No other maternal, pregnancy or neonatal outcomes are specified or reported.
Other bias	Low risk	Appears free of other bias. The authors do not report any potential conflicts of interest.