Fig. 5.

3D neuronal and spine reconstruction. A) Schematic drawing showing the pyramidal neuron structures that were analyzed using Sholl analysis (S₁…S_n; concentric spheres per distance from the soma): apical dendrite, collateral dendrites, basal dendrites. Nodes are indicated with a red circle and dendritic spines are not represented. B–M) 3D dendritic reconstruction for spine morphology analysis. B–E) Main apical, F–I) apical collateral, and J–M) dendritic segments from LY-injected pyramidal neurons in SSCx from (B, F, J) sham-operated and (D , G, K) tMCAo mice. C, E, H, I, L and M) The same dendritic segments as in B, D, F, G, J, and K, respectively, showing the 3D spine reconstruction process. Scale bar shown in M indicates 3 μm in B–M.