Extended Data Table 1:
Crystallography Data Collection and Refinement Statistics.
| METTL1-WDR4 (PDB-ID: 8D58) |
METTL1-SAM (PDB-ID: 8D59) |
METTL1-SAH (PDB-ID: 8D5B) |
|
|---|---|---|---|
| Data collection | |||
| Space group | P21 21 21 | P61 | P61 |
| Cell dimensions | |||
| a, b, c (Å) | 79.49, 91.11, 122.83 | 86.02, 86.02, 65.57 | 86.03, 86.03, 65.48 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 120.0 | 90.0, 90.0, 120.0 |
| Wavelength (Å) | 0.9791 | 0.9789 | 0.9789 |
| Resolution (Å) | 42.71- 2.45 (2.49- 2.45) * | 37.25- 2.25 (2.29- 2.25) * | 37.25- 1.93 (1.96- 1.93) * |
| R merge | 0.242 (3.76) | 0.292 (1.89) | 0.242 (3.22) |
| I/σI | 17.48 (0.8) | 13.5 (1.2) | 16.24 (1.3) |
| CC1/2 | 0.998 (0.319) | 0.983 (0.414) | 0.996 (0.450) |
| Completeness (%) | 99.9 (100.0) | 99.2 (91.6) | 100.0 (99.9) |
| Redundancy | 11.9 (8.2) | 15.5 (5.7) | 18.1 (10.5) |
| Refinement | |||
| Resolution (Å) | 42.71- 2.47 | 37.25- 2.26 | 37.25- 1.93 |
| No. reflections | 32035 | 12757 | 20756 |
| Rwork / Rfree | 0.203 / 0.229 | 0.207 / 0.229 | 0.180 / 0.212 |
| No. atoms | |||
| Protein | 4320 | 1601 | 1620 |
| Ligand/ion | 20 | 49 | 28 |
| Water | 60 | 53 | 144 |
| B-factors | |||
| Protein | 64.87 | 53.05 | 35.80 |
| Ligand/ion | 78.27 | 68.69 | 63.84 |
| Water | 60.05 | 52.39 | 44.00 |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.002 | 0.002 | 0.006 |
| Bond angles (°) | 0.436 | 0.546 | 0.951 |
| Ramachandran agreement | |||
| favored (%) | 97.79 | 98.97 | 99.48 |
| allowed (%) | 2.21 | 1.03 | 0.52 |
| outliers (%) | 0.00 | 0.00 | 0.00 |
*
Values in parentheses are for the highest resolution shell.