Table 2.

Statistics of ONT multiplex sequencing data for each group of isolates tested from 24 h of sequencing.

Group No1.	Total clean data yield in 24 h (Gbp)	Mean read length (bp)	Mean quality score	Number of reads	Mean read length N50
1	6.70	8,527	11.50	786,309	17,240
2	6.17	8,842	11.70	697,460	16,854
3	7.19	8,240	12.10	872,570	15,974
4	6.68	8,747	11.20	873,088	17,622
5	7.19	8,772	11.50	819,834	16,281
6	5.31	9,253	12.10	573,709	17,183
7	5.90	9,205	11.80	640,597	16,709
8	7.64	9,629	11.90	693,913	17,290
9	5.78	8,431	12.00	685,709	15,234
10	7.20	10,475	12.00	687,001	19,509
11	8.34	8,486	11.90	982,440	15,686
12	5.68	11,081	11.90	512,726	19,932
13	7.79	10,304	11.70	756,070	18,957
14	4.51	9,475	11.70	476,383	17,348
Average	6.58	9,248	11.79	718,415	17,273

¹Each group contains five isolates, each group of isolates were multiplexed and sequenced on one ONT flow cell.