Fig. 4. Shear stress improves epithelial integrity. A. Epithelial cells (F-actin, phalloidin) 14 days after seeding the organoids over the collagen scaffold containing mouse intestinal fibroblasts with fluid shear stress (right panel, dynamic conditions) and without shear stress (right panel, static conditions). Top view, maximal projection. Scale bar, 150 μm. B. Percentage of units covered with epithelial cells after 14 days after seeding the organoids on collagen scaffolds containing mouse intestinal fibroblasts under static conditions (stat) and with fluid shear stress (dynamic conditions, Dyn). n = 128 units, N = 3 independent experiments. Mean ± SEM, t-test ***, p < 0.0001.