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Gel shift assay demonstrates a protein-nucleic acid complex specific to the risk allele of rs10833518. Nuclear extract was obtained from U-937 histiocytic lymphoma cells and complexed with 35-bp radiolabeled probes for both alleles of the highest-association risk SNPs, rs7104612 and rs10833518. S.C., specific competitor, an unlabeled probe of the same sequence at 50× concentration. N.S.C., non-specific competitor, an unlabeled probe of scrambled sequence at 50× concentration.
