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. 2023 Feb 1;14:1101488. doi: 10.3389/fimmu.2023.1101488

Figure 4.

Figure 4

Gene expression analysis of candidate gene knock-down implicates HTATIP2 as a T cell regulator. Primary CD4+ T cells were electroporated with siRNA pools for the genes FANCF, PRMT3, SVIP, HTATIP2, or a non-targeting control siRNA in biological duplicates. Poly-A-tailed mRNA was subjected to transcriptome sequencing. (A) Principal components analysis of the ten samples color-coded by the siRNA. (B) Knock-down of HTATIP2 using siRNA in primary CD4+ T cells from four donors. mRNA levels of the indicated genes were assayed by RT-qPCR and normalized to the mRNA level of the non-targeting siRNA-transfected cells for each donor. ns, non-significant; *P < 0.05, **P < 0.01, ***P < 0.001.