Skip to main content
. 2023 Feb 1;14:1101488. doi: 10.3389/fimmu.2023.1101488

Figure 6.

Figure 6

Knock-down or overexpression of HTATIP2 modifies T cell receptor signaling, proliferation, and viability. (A) siRNA-transfected CD4+ cells were activated with different concentrations of antibodies to T cell receptor CD3/CD28 chains and stained for CD69 expression (representative experiment). The percentage of CD69-positive cells at each concentration is shown for HTATIP2 knock-down and non-targeting control. (B) Summary of results from four trials with unique donors of the experiment in panel (A). Activator concentration is 1:100. (C) CD4+ cells from five donors were transfected with siRNA and seeded at 600,000 cells/ml. The cells were counted 72 h post-transfection. (D) CD4+ cells from a human donor were transfected with in-vitro transcribed mRNA for the control E coli β-lactamase gene or for HTATIP2. After electroporation, the cells were seeded in either fresh complete RPMI medium, or 50% spent medium from activated T cells. The cells underwent bioluminescent ATP assay at 72 h post-transfection. **P < 0.01, ****P < 0.0001. ns, non-significant.