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. 2023 Jan 24;132(4):432–448. doi: 10.1161/CIRCRESAHA.122.322096

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© 2023 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 1. — Effect of Mmp12 deletion on Ang II–induced abdominal aortic aneurysms (AAA) development and progression in Apoe-/- mice. A, Kaplan-Meier survival curves of Ang II–induced AAA in Apoe^-/- (n=66) and Mmp12^-/-/Apoe^-/- (n=36) mice. The curves were compared using log-rank (Mantel-Cox) test; P<1x10^-4. B through D, Flow cytometric evaluation of CD45⁺ cells (B), CD11b⁺/Ly6G^- mononuclear myeloid cells, primarily macrophages (C), and CD11b⁺/Ly6G⁺ neutrophils (D) as % among live cells in aortas of Apoe^-/- and Mmp12^-/-/Apoe^-/- mice after 18 to 24 hours of Ang II or saline (control) infusion. Values are expressed as means±SD; n=4 to 9 per group; ns, not significant. E and F, Representative images of immunofluorescence staining and quantification of neutrophils (Ly6G, red), neutrophil extracellular traps (Cit-H3, red), and NE (red) in surviving Apoe^-/- and Mmp12^-/-/Apoe^-/- mice after 7 days of Ang II infusion (E) and control animals (F). Nuclei are stained blue with 4′,6-diamidino-2-phenylindole (DAPI). n=4 per group. Scale bar: 500 µm. *P<0.05 by Kruskal-Wallis with Dunn multiple comparisons post hoc test (B–D) or by 2-tailed Mann-Whitney U test (E and F). Ang II indicates angiotensin II; Cit-H3, citrullinated histone 3; DAPI, 4′,6-diamidino-2-phenylindole; MMP, matrix metalloprotease; and NE, neutrophil elastase.