Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 24;132(4):432–448. doi: 10.1161/CIRCRESAHA.122.322096

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — Effect of Mmp12 deletion on aortic wall complement deposition. A and B, Representative images of immunofluorescence staining and quantification of C3 deposits (red) in suprarenal abdominal aorta of Apoe^-/- and Mmp12^-/-/Apoe^-/- (A), and WT and Mmp12^-/- (B) mice. Nuclei are stained with DAPI in blue. n=5 per group. Scale bar: 500 µm. C and D, Plasma C3 and C5a levels of Apoe^-/- and Mmp12^-/-/Apoe^-/- (C), and WT and Mmp12^-/- (D) mice. n=5 to 10 per group. E and F, Plasma C3 levels in Mmp12^-/-/Apoe^-/- (E) and Mmp12^-/- (F) mice at baseline and 9 days of treatment with IgG-FH_1-5 (factor H-immunoglobulin G) or MOPC antibody (control). n=5 per group. G and H, Representative images of immunofluorescence staining and quantification of C3 (red) in suprarenal abdominal aorta of Mmp12^-/-/Apoe^-/- (G) and Mmp12^-/- (H) mice after 9 days of treatment with IgG-FH_1-5 or MOPC antibody (control). n=5 per group; Scale bar: 500 µm. I and J, Representative images of immunofluorescence staining and quantification of Cit-H3 (red) in suprarenal abdominal aorta of Mmp12^-/-/Apoe^-/- (I) and Mmp12^-/- (J) mice after 9 days of treatment with IgG-FH_1-5 or MOPC antibody (control). n=4 to 5 per group. Scale bar: 500 µm. *P<0.05, **P<0.01, ****P<1x10^-4 by 2-tailed Mann-Whitney U test (A through J). Cit-H3 indicates citrullinated histone H3; C3, complement component 3; C5a, complement component 5a; DAPI, 4′,6-diamidino-2-phenylindole; IgG-FH_1-5, factor H-immunoglobulin G; MMP, matrix metalloprotease; and WT, wild-type.