Table 3.
Apparent kinetic parameters of dihydropirimidinases from different organisms with the substrate 5,6-dihydrouracil.
| Kmapp (mM) |
apparent specific activity (µmol min-1 mg-1) |
kcatapp (s-1) |
kcatapp/Kmapp (s-1 M-1) |
Organism |
|---|---|---|---|---|
| 0.008 | 10.3 | 9.7 | 1.2 x 106 | Bos taurus 1* |
| 0.019 | 14.8 | 13.3 | 7.0 x105 | Rattus norvegicus2** |
| 0.30 ± 0.04 | 1.10 ± 0.05 | 1.07± 0.04 | 3.55 x 103 |
Oryza sativa3** (OsDHP-T1) |
1 Brooks et al. (1983). 2 Kikugawa et al. (1994). 3This work. *Activity was measured at 30°C. **Activity was measured at 37°C.