Table 4.
Apparent kinetic parameters of ß-ureidopropionases from different organisms with the substrate ß-ureidopropionic acid.
| Km app (mM) | apparent specific activity (µmol min-1 mg-1) | kcat app (s-1) | kcat app/Km app (s-1 M-1) | Organism |
|---|---|---|---|---|
| 0.008 | 0.88 | 0.65 | 8.1 x 104 | Rattus norvegicus 1 |
| 0.019 | – | 0.31 | 1.6 x 104 | Homo sapiens 2 |
| 0.011 | – | – | – | Zea mays 3* |
| 0.006 | 0.47 | 0.37 | 6.1 x 10 4 | Arabidopsis thaliana 4** |
| 2.1 | – | 26 | 1.2 x 10 4 | Agrobacterium tumefaciens 5* |
| 0.14 ± 0.01 | 3.12 ± 0.08 | 2.54 ± 0.07 | 1.81 x 104 | Oryza sativa 6** |
1 Traut (2000), 2 Maurer et al. (2018). 3 Walsh et al. (2001), 4 Andersen et al. (2008), 5 Martínez-Gómez et al. (2009), 6This work. *Activity was measured at 30°C. **Activity was measured at 25°C. The mammalian enzyme activities were measured at 37°C. The symbol “-“ indicates that data are not available.