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. 2023 Feb 1;14:1079778. doi: 10.3389/fpls.2023.1079778

Table 4.

Apparent kinetic parameters of ß-ureidopropionases from different organisms with the substrate ß-ureidopropionic acid.

Km app (mM) apparent specific activity (µmol min-1 mg-1) kcat app (s-1) kcat app/Km app (s-1 M-1) Organism
0.008 0.88 0.65 8.1 x 104 Rattus norvegicus 1
0.019 0.31 1.6 x 104 Homo sapiens 2
0.011 Zea mays 3*
0.006 0.47 0.37 6.1 x 10 4 Arabidopsis thaliana 4**
2.1 26 1.2 x 10 4 Agrobacterium tumefaciens 5*
0.14 ± 0.01 3.12 ± 0.08 2.54 ± 0.07 1.81 x 104 Oryza sativa 6**

1 Traut (2000), 2 Maurer et al. (2018). 3 Walsh et al. (2001), 4 Andersen et al. (2008), 5 Martínez-Gómez et al. (2009), 6This work. *Activity was measured at 30°C. **Activity was measured at 25°C. The mammalian enzyme activities were measured at 37°C. The symbol “-“ indicates that data are not available.