Degree of labeling (DoL) for the 3 modified PSI used in this study. The values were determined by UV/vis spectroscopy in buffer D and are stated as dyes per PSI trimer (n.a. – not applicable).
Construct | DoL (ATTO 590) | DoL (ATTO 532) |
---|---|---|
PSI-ATTO590 | 11 | n.a. |
PSI-ATTO532 | n.a. | 12 |
PSI-ATTO-mix | 8 | 11 |