Fig. 3.

Effect of Ulva prolifera polysaccharide on lipogenic protein expression in high-fat diet-fed C57BL/6 mice.

Western blot analysis of proteins involved in lipid metabolism in visceral adipose tissues. The proteins analyzed are involved in adiponectin, phosphorylated 5′AMP-activated protein kinase (p-AMPK)/AMPK), adipogenesis (peroxisome proliferator activated receptor γ [PPARγ], CCAAT), lipogenesis (acetyl-CoA carboxylase [ACC], Sterol regulatory element-binding protein 1c [SREBP1c]), lipolysis (hormone-sensitive lipase [HSL]), and β-oxidation (peroxisome proliferator activated receptor α (PPARα); carnitine palmitoyltransferase-1 [CPT-1]). For quantification of protein expression by western blotting, β-actin was used as the loading control. Data are expressed as mean ± SD (n = 7–8 mice/group). The statistical significance of differences among the five groups were analyzed by one-way ANOVA and Duncan’s multiple range tests. The values with different letters (a–c) are significantly different (p < 0.05) between each group.