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. 2023 Feb 3;19(2):e1010621. doi: 10.1371/journal.pgen.1010621

Fig 6. RPG interact CERBERUS at the TGN/EE compartment.

Fig 6

(A-B) GFP-RPG (green) and TGN/EE marker SYP61-mCherry (magenta) were co-expressed in N. benthamiana leaf cells (A) or L. japonicus root protoplasts (B). (C) Co-expressed RPG-CERBERUS BiFC construct (yellow) and NLS-DsRed (magenta) in N. benthamiana leaf cells. Plots (A‴-C‴) show fluorescence intensities of the regions of interest (indicated by white line in [A″-C″]). (D) Confocal images of SPPR1-GFP in L. japonicus WT, rpg-1 and cerberus-12 transgenic roots inoculated with M. loti R7A/lacZ 2 days after Brefeldin-A induced BFA body formation. E, Quantification of BFA bodies size in WT, rpg-1 and cerberus-12 expressing SPPR1-GFP after 2 h BFA treatment. Asterisks indicate significant differences (Students t-test), between mutants and WT (error bars represent SE). Scale bars: 25 μm (A, C); 20 μm (B); 200 μm (D).