Figure 4.

NLE1 regulated non−small−cell lung cancer via the Akt signaling pathway. (A) KEGG pathway analysis was performed using genes whose expression was increased or decreased by NLE1 silencing in PrimeView human gene expression array. (B, C) The cell proliferation levels (B) and apoptosis levels (C) of shCtrl and shNLE1 infected A549 and NCI-H1299 cells were tested after treating with 10 μM SC79. (D) The expression of AKT and p-AKT in shCtrl and shNLE1 A549 and NCI-H1299 cells was detected by western blot after treating with 10 μM SC79. The data are expressed as mean ± SD (n ≥ 3). ** P < 0.01, *** P < 0.001, *P < 0.05.