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. 2023 Feb 15;9(7):eade8939. doi: 10.1126/sciadv.ade8939

Fig. 3. Temporal progression of VM-like phenotypes in PIK3CAE542K vascular networks.

Fig. 3.

(A) Time-lapse confocal images (maximum intensity projections) of GFP control endothelial cells (cyan) cultured in fibrin ECM (magenta) and corresponding volume rendering of segmented vascular networks. Scale bars, 100 μm. (B) Time-lapse images (single-plane and maximum intensity projections) of PIK3CAE542K cells (cyan) cultured in fibrin ECM (magenta) (top), maximum intensity projections of fibrin ECM (middle), and corresponding 3D rendering of segmented ECM void spaces. Scale bars, 100 μm.