Figure 7.

Rh Bri2 BRICHOS R221E mitigates astrogliosis and microgliosis and reduces colocalization of Aβ and GFAP in App^NL-F mice

(A–I) Representative images of brain sections stained with anti-GFAP antibody (A and C) and histograms showing GFAP-positive areas in the hippocampus and cortex with n = 5 mice/group, 4 histological sections per mouse (B). Shown are representative merged images of brain sections double stained using anti-Aβ and anti-GFAP antibodies and counterstained with Hoechst nuclear stain (D and F) and histograms representing percent colocalization of Aβ and GFAP in hippocampus and cortex regions with n = 4 mice/group, 4 histological sections per mouse (E). White arrows in (D) identify examples of yellow (i.e., Aβ and GFAP colocalized) spots. Also shown are representative images of brain sections stained with anti-Iba1 antibody (G and I) and histograms showing Iba1-positive areas in the hippocampus and cortex (n = 4–5 mice/group, 4 histological sections per mouse) (H). Data are shown as mean ± SEM. Unpaired parametric two-tailed t test was used to calculate the p value for all analyses. Scale bars represent 500 μm (A, D, and G) and 20 μm (C, F, and I). ∗p < 0.05, ∗∗∗p < 0.001.