Extended Data Fig. 7. CXCL12-Atz is cross-reactive to mouse cell lines and stable in vivo.
Flow cytometry showing that human CXCL12 is cross reactive and binds to the surface of a, MC38 and b, CT26 mouse cell lines. c, Dose escalation showing mouse PD-L1 degradation in MC38 and CT26 cells following 24 hr treatment with CXCL12-Atz. Representative western blot of dose escalation showing mouse PD-L1 degradation in d, MC38 or e, CT26 mouse cells following 24 hr treatment with CXCL12-Atz and mouse IFNg. f, Representative western blot showing plasma levels of CXCL12-Tras in male nude mice injected intravenously with 5, 10, or 15 mg/kg. Data are representative of three independent biological replicates or mice. Densitometry was used to calculate protein levels and normalized to PBS control. Data are represented as mean values and error bars represent the standard deviation of biological replicates.