Extended Data Fig. 9. KineTACs mediate the uptake of extracellular TNFa.
a, Representative flow cytometry showing shift in median fluorescence intensity in HeLa cells treated for 24 hr with 50 nM CXCL12-Ada and 25 nM TNFa-647 compared to TNFa alone. b, Summary of flow cytometry demonstrating significant uptake of TNFa-647 in HeLa cells following 24 hr treatment with 50 nM CXCL12-Ada compared to adalimumab isotype (P = 0.0072). Adalimumab isotype showed no significant uptake of TNFa-647 compared to TNFa-647 alone (P = 0.8701). N = 3 biologically independent experiments. c, HeLa cells treated for 24 hr with varying ratios of CXCL12-Ada to TNFa, at constant 25 nM TNFa-647, demonstrate that increasing the KineTAC:TNFa ratio increases TNFa uptake. Data are represented as mean values and error bars represent the standard deviation of biological replicates. P-values were determined by one-way ANOVA with Sidak’s multiple comparisons test. Fold changes are reported relative to incubation with soluble ligand alone.