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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Glia. 2022 Dec 8;71(4):926–944. doi: 10.1002/glia.24315

Figure 3. Kir4.1-tdTomato mice given TMX at post-weaning stages exhibit tdTom expression in TPSC of the diaphragm as well as in some cells of the phrenic nerve.

Figure 3.

A, Low-magnification images of the diaphragm muscle from P50 Kir4.1-tdTomato mice given TMX from P30-P43 show tdTom+ TPSC at AlexaFluor 488-conjugated α-BTX-labeled AChR clusters (arrowhead), but also in cells associated with phrenic nerve (arrow). B, Diaphragm muscle from P25 Kir4.1-tdTomato mice given TMX from P9-P22 shows tdTomato expression (red) by two Hoechst-labeled nuclei (arrows, purple) near AlexaFluor 488-conjugated α-BTX-labeled AChR clusters (green) in the left and middle panel. Percentage of TPSC at α-BTX-labeled NMJ expressing tdTom in the diaphragm and EDL muscles after TMX from P30-P43 (right panel); data in text.