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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Glia. 2022 Dec 8;71(4):926–944. doi: 10.1002/glia.24315

Figure 9. Kir4.1-tdTomato mice given TMX at post-weaning stages exhibit tdTom expression in non-myelinating Schwann cells (NMSC) of autonomic nerves and enteric glial cells (EGC) of myenteric ganglia.

Figure 9.

A, L1+ NMSC, but not MPZ+ MSC, of the parasympathetic cervical vagus nerve trunk express tdTomato when TMX was given at P30-P43. B, Cells of the non-myelinating sympathetic splenic nerve, identified just outside the spleen in sections by its association with tyrosine hydroxylase (TH), NF-M double-positive axons, express tdTom when TMX was given at P30-P43. C, S100β-positive EGC within the myenteric ganglia of the colon express tdTom when TMX was given at P30-P43, as shown in low- and high-magnification images (top row, left and right two panels, respectively). tdTom was also expressed by S100β-positive EGC associated with the axons of these myenteric neurons as they course through the colonic smooth muscle (bottom row, left two panels). tdTom was not expressed by NeuN+ myenteric neurons (bottom row, right two panels). D, tdTom was expressed by S100β-positive EGC of the mucosa (arrows) but also expressed by a population of S100β-negative cells (asterisks; left two panels). Percentage of L1+ autonomic non-myelinating Schwann cells (A NMSC) of the vagus nerve (VN; 92.3 ± 7%; n=4; 2M,2F; c=12/n) or the splenic nerve (SpN; 96.8 ± 2.7%; n=4; 2M,2F; c=7/n) that express tdTom after TMX from P30-P43 (middle right panel). Percentage of S100β+ myenteric EGC of the enteric nervous system / ENS (EGC; 87.5 ± 8.7%; n=4; 2M,2F; c=54/n) or NeuN+ myenteric neurons (MyN: 1.25 ± 1.5%; n=4; 2M,2F; c=48/n) that express tdTom after TMX from P30-P43 (far right panel).