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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nat Genet. 2023 Jan 12;55(2):346–354. doi: 10.1038/s41588-022-01278-7

Fig. 5. scMPRA recapitulates Gnb3 expression patterns.

Fig. 5.

(a) The expression of the wild-type Gnb3 promoter in scMPRA reflects endogen]ous expression levels of Gnb3 in the respective cell types. The solid line represents the best fit linear regression. (b) The expression of the entire Gnb3 library (n=115 variants) in different cell types also follows endogenous Gnb3 expression (****: p-value < 0.0001, two-sided Mann-Whitney U test). (c) scMPRA recapitulates the effects of a known Gnb3 variant, where the CRX3Q50/CRX5Q50 variant reduces expression in bipolar cells specifically (*: p-value < 0.05, two-sided Welch’s t-test). All expression values are plotted as the mean of two biological replicates.