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. 2023 Feb 15;13:2692. doi: 10.1038/s41598-023-29817-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Both the AcrB and AcrA components contribute to survival of LF82 within macrophages. Intracellular LF82 (black square), LF82 ΔacrB (open triangle), and LF82 ΔacrA (open diamond) bacteria were recovered from THP-1 differentiated into macrophages at 0, 1, 2, 3, 4 and 5 h p.i. (referred to as T0, T1, T2, T3, T4 and T5, respectively) and stained with DAPI/PI. The values are expressed as percentage of PI (+) dead bacteria relative to DAPI (+) bacteria. The results are the average of at least three independent experiments. Error bars represent the SD. The statistical significance was determined by a two tailed student’s t test. *p ≤ 0.01.