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. 2023 Feb 15;13:2681. doi: 10.1038/s41598-023-29649-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Non-linear multimodal images of healthy (A) and inflamed (B) colonic mucosa with a combination of CARS @ 2850 ${cm}^{- 1}$ (Ai and Bi), TPEF @ 458bp64 (Aii and Bii), and SHG @ 415bp3 (Aiii and Biii). The composite images contain all three channels (Aiv and Biv), and the inset shows zoomed-in regions of interest, which are indicated by white dashed squares. The arrowhead labels are indicated below the images.