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. 2023 Feb 2;13:1096800. doi: 10.3389/fpls.2022.1096800

Figure 1.

Figure 1

Infection of Arabidopsis thaliana with Pst/AvrRpm1 induces the local accumulation of BETA-D-XYLOSIDASE 4 (BXL4) transcripts. Col-0, bxl4-1, and bxl4-3 plants were inoculated by syringe infiltration with 105 cfu/mL of Pst or Pst/AvrRpm1 (A/C/D) or by spray treatment with 108 cfu/mL of Pst or Pst/AvrRpm1 (B/E/F). (A, B) The BXL4 transcript abundance in the inoculated leaves was determined by qRT-PCR two (T2) or three days (T3) later. Transcript accumulation was normalized to that of UBIQUITIN and is shown relative to the normalized transcript levels in the appropriate Col-0 mock (M) controls. Black dots represent biologically independent data points and horizontal lines represent mean values ± SD from three to four biologically independent replicate experiments. The letters above the scatter dot plots indicate statistically significant differences (one-way ANOVA and Tukey’s test, P=<0.05, for (BXL4: infiltration T2): n=3, F(5, 12)=52.31; for (BXL4: spray T3): n=3-4, F(8, 24)=125.8). (C–F) In planta titers of Pst and Pst/AvrRpm1 were determined at the time points indicated below the panels at T0: two hours post inoculation/T1: one day post inoculation (dpi), T2: two dpi, and T4: four dpi. Box plots represent average titers from four to seven biologically independent experiments, including at least three replicates each ± min and max values. Different letters above the box plots indicate statistically significant differences for single means (one-way ANOVA and Tukey’s tests for P=<0.05, for (C): F(8, 136)=144.3, Col-0 T0 n=15, Col-0 T2 n=17, Col-0 T4 n=22, bxl4-1 T0 n=15, bxl4-1 T2 n=18, bxl4-1 T4 n=22, bxl4-3 T0 n=12, bxl4-3 T2 n=12, bxl4-3 T4 n=12; for (D): F(8, 137)=361.6, Col-0 T0 n=15, Col-0 T2 n=18, Col-0 T4 n=22, bxl4-1 T0 n=15, bxl4-1 T2 n=18, bxl4-1 T4 n=22, bxl4-3 T0 n=12, bxl4-3 T2 n=12, bxl4-3 T4 n=12); for (E): F(8, 132)=285.3, Col-0 T1 n=21, Col-0 T2 n=27, Col-0 T4 n=32, bxl4-1 T1 n=20, bxl4-1 T2 n=27, bxl4-1 T4 n=32, bxl4-3 T1 n=9, bxl4-3 T2 n=12, bxl4-3 T4 n=12; for (F): F(8, 164)=169.7, Col-0 T1 n=18, Col-0 T2 n=24, Col-0 T4 n=28, bxl4-1 T1 n=18, bxl4-1 T2 n=24, bxl4-1 T4 n=28, bxl4-3 T1 n=9, bxl4-3 T2 n=12, bxl4-3 T4 n=12).