Figure 5.

Exogenous xylose induces EDS1- and LLP1-dependent systemic defense against Pst. Plants of the genotypes Col-0, bxl4-1, eds1-2, and llp1-1 were inoculated with a mixture of D- and L-xylose, termed D-/L-xylose (10 µM (X) or another dose as indicated below the panels), or with a corresponding mock treatment (M or mock as indicated below the panels). Three days later, two distal leaves were challenged with 10⁵ cfu/mL of Pst and monitored for in planta bacterial titers 4 days after. Box plots represent average Pst titers of 12 (A) or seven (B) biologically independent experiments ± min and max value. Different letters above box plots indicate statistically significant differences for means (one-way ANOVA and Tukey’s test for P=<0.05, for (A, Col-0): F(5, 189)=46.95, mock n=46, 100 nM n=34, 1 µM n=27, 10 µM n=35, 100 µM n=27, 1 mM n=26; for (B): F(7, 284)=43.20, Col-0 M n=46, Col-0 X n=35, bxl4-1 M n=46, bxl4-1 X n=30, eds1-2 M n=41, eds1-2 X n=25, llp1-1 M n=43, llp1-1 X n=26).