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. 2023 Feb 15;14(2):125. doi: 10.1038/s41419-023-05646-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B The results of RT-qPCR (A) and western blot (B) showed the expression of NSUN2 in 143b cells. C, D The results of RT-qPCR (C) and western blot (D) showed the expression of NSUN2 in U2 cells. E–G The CCK-8 assay (E, 143b cells; F, U2 cells) and colony formation assay (G) showed that proliferation of OS cells decreased when NSUN2 was knocked down. H Transwell assay indicated that the invasion ability of OS cells decreased when the expression of NSUN2 decreased. I The results of the wound-healing assay confirmed that the cells in the sh-NSUN2 group had a lower migration ability. Statistical analysis was performed according to the data of three independent experiments.