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. 2023 Feb 15;14(2):125. doi: 10.1038/s41419-023-05646-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Venn diagram of RNA-seq showing the genes whose expression level was positively or negatively correlated with the expression level of NSUN2. The sh-NSUN2 group means the cells were stably transfected with sh-NSUN2#1. B The heatmaps made by the genes obtained from (A). C, D The results of RT-qPCR showed the expression level of FABP5 mRNA when NSUN2 was knocked down or overexpressed in 143b cells. E The results of western blot showed the expression level of FABP5 protein when NSUN2 was knocked down or overexpressed in 143b cells. F, G The results of RT-qPCR showed the expression level of FABP5 mRNA when NSUN2 was knocked down or overexpressed in U2 cells. H The results of western blot showed the expression level of FABP5 protein when NSUN2 was knocked down or overexpressed in U2 cells. I, J The results of RIP and RT-qPCR in 143b and U2 cells. IgG was used as a negative control to preclude nonspecific binding. K The result of RT-qPCR showed FABP5 was highly expressed in OS tissues. The result of RT-qPCR was statistical analyzed according to the data of three independent experiments.