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. 2023 Jan 13;26(2):105963. doi: 10.1016/j.isci.2023.105963

Figure 7.

Figure 7

MiR-218-5p targeted to TNFAIP3

(A) Targeted sequence prediction of miR-218-5p in RNA22.

(B) Immunoblots (left) indicated TNFAIP3 expression was significantly repressed in CFs transfected with miR-218-5p.

(C) Luciferase assay of miR-218-5p and TNFNAIP3 5′UTR region. Co-transfection of miR-218-5p + WT sequence significantly reduced luciferase activity compared with the scramble CTL + WT. One-way ANOVA followed by Tukey’s post hoc test, ∗∗ = p< 0.01; NS = nonsignificant.

(D) Representative immunoblots of CFs treatment.

(E) Quantitation of expression of TNFAIP3, Col1a, Col3a, and CTGF. One-way ANOVA followed by Tukey’s post hoc test; ∗∗ = p<0.01, ∗∗∗ = p< 0.001. Data represented as mean ± SD.