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. 2023 Feb 15;13:2739. doi: 10.1038/s41598-023-29948-4

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of the time between blood collection and plasma preparation, gender and age on the BTC index. (A) 6 HI blood samples were separated into 4 aliquots and the plasmas were prepared 4, 24, 48 and 72 h later (one spot color per individual). (B) The BTC index is not different between women (F) and men (M) (healthy donors). (C) The BTC index is not different between healthy individuals younger or older than 50 years.