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. 2023 Feb 15;14(2):124. doi: 10.1038/s41419-023-05648-9

Fig. 3. ASAP1 promotes GC cell proliferation in vitro and xenograft tumor growth in vivo.

Fig. 3

A, D, G Western blot analysis of ASAP1 of MGC-803 and SGC-7901 cells expressing ASAP1-HA, depleted of ASAP1 by siRNA or partially knocked out of ASAP1 by CRISPR-Cas9. B, E, H CCK-8 assays with MGC-803 and SGC-7901 cells expressing ASAP1-HA, depleted of ASAP1 by siRNA or partially knocked out of ASAP1 by CRISPR-Cas9. C, F, I Representative images from colony formation with MGC-803 and SGC-7901 cells expressing ASAP1-HA, depleted of ASAP1 or partially knocked out of ASAP1. J, K Cell cycle assay of SGC-7901 cells expressing ASAP1-HA or partially knocked out of ASAP1. L, M Apoptosis assay of SGC-7901 cells expressing ASAP1-HA or partially knocked out of ASAP1. N In vivo analysis using a tumor xenograft model. The growth curve of xenograft tumors from WT (n = 5) and ASAP1 ± (n = 5) SGC-7901 cells. ns not significant; *p < 0.05; **p < 0.01; ***p < 0.001.