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. 2023 Feb 15;13:2729. doi: 10.1038/s41598-023-29657-y

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Consensus alignment of reference COXI sequences used for primer and probe design. Positions of forward and reverse primers are highlighted by light blue asterisks. Species-specific probes are highlighted in teal, with single-nucleotide polymorphisms in colour.