Figure 3.
Image analysis pipeline
Single molecule localizations of the same probe are clustered according to the DBScan algorithm. Molecules not localized to a cluster are eliminated from further analysis. Cluster α shapes are determined and further analysis is conducted on gephyrin volume, vGAT-gephyrin centroid distance, and density. (Representative clusters obtained from data generated in Guzikowski and Kavalali,1 Images have been manipulated to represent potential outcomes of this protocol).