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SDCA plate with 5-FOA and Ura
| Reagent | Final concentration | Amount |
|---|---|---|
| YNB w/o AA, AS | 0.17% (w/v) | 1.7 g |
| Ammonium Sulfate | 0.5% (w/v) | 5 g |
| D-(+)-Glucose | 2%(w/v) | 20 g |
| Casamino acids | 0.5% (w/v) | 5 g |
| Agar | 2%(w/v) | 20 g |
| 5-FOA | 0.1% (w/v) | 1 g |
| Uracil stock solution | 20 mg/mL | 25 mL |
| ddH2O | – | Up to 975 mL |
Autoclave to sterilize.
5-FOA and Uracil are added after autoclave.
Store at 4°C for up to a month.
