Li-PEG
Reagent | Final concentration | Amount |
---|---|---|
Lithium Acetate Dihydrate | 100 mM | 1 g |
1 M Tris-HCl buffer, pH7.5 | 10 mM | 1 mL |
0.5 M EDTA, pH8.0 | 1 mM | 200 μL |
PEG | 50% (w/v) | 50 g |
ddH2O | – | Up to 100 mL |
Autoclave to sterilize.
Store at 25°C for up to a year.