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. 2023 Feb 2;14:1075071. doi: 10.3389/fmicb.2023.1075071

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Copyright © 2023 Islas-Morales, Cárdenas, Mosqueira, Jiménez-García and Voolstra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Light and electron microscopy observations support the presence of putative nucleolus-like domains in the crenarchaeon Saccharolobus solfataricus at the ultrastructural level. (A1) Light microscopy of the AgNOR reaction. (A2) Light microscopy of unstained S. solfataricus cells (negative control). (B1) Transmission Electron Microscopy (TEM) of S. solfataricus cells, with (B2) detail of discrete fibro-granular structures. (C1) Ultrastructural AgNOR impregnation of subcellular structures, with (C2) high magnification of AgNOR positive subcellular structures. (D1) Ultrastructural In situ hybridization (UISH) of 16S and 23S rDNA clusters, with (D2) magnified image showing an underlying electron-dense region.