Fig. 5.

In vivo pharmacodynamic assessment. (A) Flow diagram of the experimental process of arthritis induction and IA treatment in rabbits. The red inverted triangle denotes SC immunization. The blue inverted triangle denotes IA arthritis induction. The black inverted triangle denotes IA injection of CEL-gel or CEL-sol. The black star denotes the treatment endpoint that rabbits were sacrificed. The trend of joint diameter (B), and knee skin temperature (C) in the process of different treatments (n = 12). (D-E) NF-κB express levels in serum and joint from the different treatment groups. (F-G) Quantification of TNF-α level in serum and joint from the different treatment groups. (H-I) Quantification of IL-1β level in serum and joints. (J-K) Quantification of IL-6 level in serum and joints. (L-M) MMP-3 secreted level in serum and joints (n = 6). Values are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.