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. 2023 Feb 3;4(1):102088. doi: 10.1016/j.xpro.2023.102088

Figure 8.

Figure 8

Expected outcomes of protein localization using sonication, sodium carbonate or proteinase K and Triton X-100

(A–C) Mitochondria from HEK293T cells were sonicated (A) or treated with Na2CO3 (B), proteinase K or/and Triton X-100 (C), and then left untreated (M) or separated into supernatant (S) and pellet (P) and analyzed by Western blot. TOM40, TIM29, HSP60 and CytC are used as markers representing outer membrane, inner membrane, mitochondrial matrix and mitochondrial intermembrane proteins, respectively.